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pdr gfp plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pdr gfp plasmid
    Pdr Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdr+gfp/pDRGFP+(Plasmid+%2326475)/pmc12922743-240-6-8
    Average 96 stars, based on 188 article reviews
    pdr gfp plasmid - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: APOL2 Stabilizes Ku80 to Confer NHEJ‐Mediated Radioresistance in Gastric Cancer
    Article Snippet: The pCMV‐GFP‐APOL2 (WT) and pCMV‐GFP‐APOL2 (OE) plasmids were purchased from Genechem Biotechnology Co., Ltd. (Shanghai, China).

    Plasmid Preparation:

    Article Title: Targeting platinum-resistant ovarian cancer by disrupting histone and RAD51 lactylation
    Article Snippet: .. The pre-treated A2780 or OVCAR8 cells were co-transfected with pCBAScel (Addgene, plasmid no.#26477) and pDR-GFP (direct-repeat green fluorescent protein; Addgene, plasmid no.#26475) using Lipofectamine 2000 for 24 h. After an additional 24 h incubation, the cells were digested and suspended. .. The percentage of GFP + cells was determined by the flow cytometry assay in Beckman CytoFLEX FCM (Beckman Coulter, USA).

    Article Title: Targeting RAD52 overcomes PARP inhibitor resistance in preclinical Brca2 -deficient ovarian cancer model
    Article Snippet: Briefly, the reporter constructs were hprtSAGFP (Addgene, MA. .. USA, plasmid #41594; http://n2t.net/addgene:41594 ; RRID:Addgene_41594), pDR-GFP (Addgene plasmid #26475; http://n2t.net/addgene:26475 ; RRID:Addgene_26475), and pCBASceI (Addgene plasmid #26477; http://n2t.net/addgene:26477 ; RRID:Addgene_26477). ..

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: Repression of PFKFB3 sensitizes ovarian cancer to PARP inhibitors by impairing homologous recombination repair
    Article Snippet: .. In brief, control, PFKFB3-KD SKOV3 and OVCAR8 cells were transfected with 1 μg of pDR-GFP (Addgene, 26,475) along with 0.75 μg of pCBASceI plasmid (Addgene, 26,477). .. Following a 48 h incubation period post-transfection, the cells were collected for analysis using a BD FACScan flow cytometry system (Becton Dickinson, Franklin, NJ, USA).

    Article Title: Magnolol suppresses TKI-resistant EGFR-mutant lung cancer by inhibiting EGFR and AXL-cMyc.
    Article Snippet: Acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), particularly osimertinib, remains a major therapeutic challenge in EGFR-mutant lung cancer.. In this study, we evaluated the pharmacological activity of magnolol, a natural biphenolic compound, in EGFR-mutant lung cancer models, including osimertinib-resistant cells harboring tertiary mutations such as C797S.. Magnolol inhibited proliferation and induced apoptosis in both sensitive and resistant cell lines.

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: APOL2 Stabilizes Ku80 to Confer NHEJ-Mediated Radioresistance in Gastric Cancer.
    Article Snippet: siRNAs targeting si-USP7/si-Ku80 and si-NC were obtained from Miaoling Biotechnology Co., Ltd. (Wuhan, China). .. Plasmid Construction: The pCMV-GFP-APOL2 (WT) and pCMV-GFPAPOL2 (OE) plasmids were purchased from Genechem Biotechnology Co., Ltd. (Shanghai, China). pDR-GFP (#26475), hprt-SA-GFP (#41594), pim-EJ5-GFP (#44026), EJ2-GFP-puro (#44025), and I-SceIg were purchased from Addgene Co., Ltd. (USA). .. Plasmid Transfection and Viral Transduction: HEK293T cells were transfected with plasmid DNA using a Jetprime transfection kit (Polyplus, #101000046) following the manufacturer’s instructions.

    Incubation:

    Article Title: Targeting platinum-resistant ovarian cancer by disrupting histone and RAD51 lactylation
    Article Snippet: .. The pre-treated A2780 or OVCAR8 cells were co-transfected with pCBAScel (Addgene, plasmid no.#26477) and pDR-GFP (direct-repeat green fluorescent protein; Addgene, plasmid no.#26475) using Lipofectamine 2000 for 24 h. After an additional 24 h incubation, the cells were digested and suspended. .. The percentage of GFP + cells was determined by the flow cytometry assay in Beckman CytoFLEX FCM (Beckman Coulter, USA).

    Stable Transfection:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Transfection:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: Repression of PFKFB3 sensitizes ovarian cancer to PARP inhibitors by impairing homologous recombination repair
    Article Snippet: .. In brief, control, PFKFB3-KD SKOV3 and OVCAR8 cells were transfected with 1 μg of pDR-GFP (Addgene, 26,475) along with 0.75 μg of pCBASceI plasmid (Addgene, 26,477). .. Following a 48 h incubation period post-transfection, the cells were collected for analysis using a BD FACScan flow cytometry system (Becton Dickinson, Franklin, NJ, USA).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Construct:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Non-Homologous End Joining:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Control:

    Article Title: Repression of PFKFB3 sensitizes ovarian cancer to PARP inhibitors by impairing homologous recombination repair
    Article Snippet: .. In brief, control, PFKFB3-KD SKOV3 and OVCAR8 cells were transfected with 1 μg of pDR-GFP (Addgene, 26,475) along with 0.75 μg of pCBASceI plasmid (Addgene, 26,477). .. Following a 48 h incubation period post-transfection, the cells were collected for analysis using a BD FACScan flow cytometry system (Becton Dickinson, Franklin, NJ, USA).

    Expressing:

    Article Title: Magnolol suppresses TKI-resistant EGFR-mutant lung cancer by inhibiting EGFR and AXL-cMyc.
    Article Snippet: Acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), particularly osimertinib, remains a major therapeutic challenge in EGFR-mutant lung cancer.. In this study, we evaluated the pharmacological activity of magnolol, a natural biphenolic compound, in EGFR-mutant lung cancer models, including osimertinib-resistant cells harboring tertiary mutations such as C797S.. Magnolol inhibited proliferation and induced apoptosis in both sensitive and resistant cell lines.



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